Isotope Labeling of Biomolecules - Labeling Methods Elsevier Science
Publisher: Elsevier Science

A rapid and robust method for selective isotope labeling of proteins. An economical method for (15)N/(13)C isotopic labeling of proteins expressed in Pichia pastoris. Isotope labeling in mammalian cells. Two major approaches have been mainly used for segmental isotopic labeling, i.e. Segmental isotopic labeling of proteins for nuclear magnetic resonance. A simple method for amino acid selective isotope labeling of recombinant proteins in E. Doi: 10.1016/j.ymeth.2011.08.019. Isotope labeling in Biomolecular NMR. Sigma-Aldrich.com presents an informational article concerning biomolecular NMR and the use of Isotope Labeling Methods for Protein Dynamics Studies. Doi: 10.1016/S0076-6879(09)62008-5. Isotope labeling in insect cells. Two related approaches are generally used in the segmental isotopic labeling of proteins: expressed protein ligation and protein trans-splicing. Key words: heteronuclear NMR, isotope labeling, protein expression. Several strategies for efficient isotopic labeling of recombinant proteins in E.coli have been proposed [4, 19–21]. For example, isotopic labeling can be limited to a subset of amino acid types and/ or protein domains [62]. We developed two methods for stable-isotope labeling of proteins by cell-free synthesis. Tong KI(1), Yamamoto M, Tanaka T. Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris. Series: Advances in Experimental Medicine and Biology, Vol.